Reference number
ISO/TS 19006:2016
ISO/TS 19006:2016
© ISO 2024
Technical Specification
ISO/TS 19006:2016
Nanotechnologies — 5-(and 6)-Chloromethyl-2’,7’ Dichloro-dihydrofluorescein diacetate (CM-H2DCF-DA) assay for evaluating nanoparticle-induced intracellular reactive oxygen species (ROS) production in RAW 264.7 macrophage cell line
Edition 1
2016-08
2016-08
Technical Specification
ISO/TS 19006:2016
63697
Publicado
(Edición 1, 2016)
Esta publicación se revisó y confirmó por última vez en 2023.
Por lo tanto, esta versión es la actual.
Resumen
ISO/TS 19006:2016 describes how to test and evaluate results obtained from in vitro ROS generation in RAW 264.7 macrophage cells exposed to nano-objects, nanoparticles, their aggregates and agglomerates using the CM-H2DCFDA assay.
The protocol in ISO/TS 19006:2016 is limited to use of a 24 well plate so if other plates were to be used, volumes would need to be adjusted and the protocol steps validated to ensure confidence in the test results.
Informaciones generales
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Estado: PublicadoFecha de publicación: 2016-08Etapa: Norma Internacional confirmada [90.93]
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Edición: 1Número de páginas: 21
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Comité Técnico :ISO/TC 229ICS :07.120
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